Electroporation相关论文
Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fi
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Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bl
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目的 建立能够稳定表达绿色荧光蛋白(GFP)的小鼠胚胎干细胞(ESC),并诱导其向神经细胞分化,为临床移植治疗神经系统疾病提供方便追......
Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fi
Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA conta......
Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bl
Background So far, there is no efficient treatment for pulmonary fibrosis. The objective of this study was to determine ......
Genetic Transformation of Nannochloropsis oculata with a Bacterial Phleomycin Resistance Gene as Dom
The gene ble from Streptoalloteichus hindustanus is widely used as a selective antibiotic marker.It can control the phle......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
目的 探索以绿色荧光蛋白(GFP)为报告基因的重组质粒pEGFP/SDF-1a/54/KDEL电穿孔悬浮培养的Molt-4细胞的高效转染条件.方法 通过控......
目的 将戊型肝炎病毒ORF3基因转染HeLa细胞,建立能稳定表达ORF3蛋白(pORF3)的细胞株.方法 将ORF3基因片段克隆至真核表达质粒pcDNA......
陡脉冲不可逆性电击穿是一种新的肿瘤物理治疗方法.由于其独特的作用机制,不仅能有效杀灭肿瘤,且具有无热效应、操作简单、治疗时......
OPTIMIZATION OF ELECTROPORATION PARAMETERS FOR TRANSFECTION OF PLASMID DNA INTO MURINE BONE MARROW-D
Objective To explore the optimal electroporation parameters for transfection of plasmid DNA into murine bone marrow-deri......
Study of the selective effect on cells induced by nanosecond pulsed electric field with the resistor
A resistor-capacitor (RC) circuit model is proposed to study the effect of nanosecond pulsed electric field on cells acc......
目的 分离培养大鼠胎脑神经干细胞(NSC),研究电穿孔技术在NSC中的转染效率.方法 从sD胎鼠脑组织中分离、培养和扩增NSC,利用免疫荧......
10-23脱氧核酶(10-23 DNAzyme,10-23DZ)是近年来利用体外分子进化技术筛选得到的一种具有催化功能的单链DNA分子,能高效、特异催化......
目的 探索电穿孔介导的基因治疗对下颌骨DO过程中牵引间隙新生骨密度与强度的影响,从而为促进下颌骨DO新骨生成,缩短牵引周期,减少......
目的 探索电穿孔介导的基因治疗对兔下颌骨牵引成骨过程中细胞周期调节蛋白表达的影响。方法 45只新西兰大白兔，双侧下颌骨截骨后3d......
目的 研究不同剂量结核病DNA疫苗电转染后的免疫原性.方法 40只BALB/c小鼠通过随机数字表法分为8组,每组5只.其中4组分别用100 μl......
幼胚的遗传转化对研究植物胚胎发育相关基因的表达与调控具有重要意义, 也为植物遗传改良提供新的技术.本研究借助一种自制的特殊......
目的 探讨非病毒载体电穿孔(electroporation,EP)对大鼠早期骨性关节炎的基因治疗效果.方法 选用8周龄健康级雄性SD大鼠,通过切断......
香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源.但近年来,香蕉生产受到严重的病虫危害.大多数香蕉栽培品种是三倍体,......
应用弱正弦波电磁场改变细胞膜的穿透性并引起其失活和坏死.研究中,将人类癌细胞U-937和K-562放置于强度为10 mT和39 mT(50 Hz)的......
为探讨ns脉冲电场诱导细胞器膜产生电穿孔的机理,采用分子动力学软件从原子水平上分析了ns脉冲电场作用下细胞脂双层膜电穿孔形成......
It is first reported that plant young proembryos expressed exogenous reporter genes by electroporation. Young proembryos......
Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthes
A procedure for transformation of intact Streptococcus zooepidemicus cells by electroporation was developed through a sy......
